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Cells were grown to 85%95% confluence before they were placed in a humidified 37 C incubator, and cells from passages 520 were used

The jump from 10% to 26.5% regrowth (p = 0.0025) with just 2 additional sessions suggests the biological response continues to accumulate, raising the question of whether 6-8 sessions could produce even greater results

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This in turn can make the cell less able to defend itself against futher free radicals, until eventually the cells P-53 gene bank is activated to destroy itself